Dr Anjana C. Karawita

Dr Anjana C. Karawita

Senior Research Scientist · Veterinary Pathologist

Australian Centre for Disease Preparedness (ACDP), CSIRO, Geelong
Adjunct Senior Research Fellow, James Cook University

Professional Summary

I am a Senior Research Scientist and veterinary pathologist at CSIRO’s Australian Centre for Disease Preparedness (ACDP), with a distinctive dual expertise in veterinary anatomic pathology and genomics/bioinformatics — a combination that is rare both nationally and internationally. I bring over a decade of experience spanning diagnostic pathology, infectious disease research, comparative genomics, and high-containment laboratory operations.

At CSIRO, I lead research at the interface of pathology and pathogen biology, investigating the genesis, pathogenesis and host responses to emerging infectious diseases in wildlife and domestic species, with particular depth in avian influenza, Japanese encephalitis virus, lyssaviruses, and SARS-CoV-2. My work integrates whole-genome and transcriptomic analysis with classical pathological investigation to deliver insights that neither discipline could achieve alone.

I am actively progressing toward board certification as a Diplomate of the American College of Veterinary Pathologists (ACVP), having passed Phase I of the ACVP Board Examination. I have performed over 1,000 post-mortem examinations across domestic and wild species, and I am trained and certified to operate in Physical Containment Level 3 (PC3) facilities.

Beyond the bench, I serve as a member of the ACDP Academic Board, supervise two PhD students and multiple Honours and undergraduate research students, and have secured over $520,000 in competitive grant funding as lead or co-lead investigator, with additional internal grants and a pending external application in environmental biosecurity. I have contributed to national capacity development in the Northern Territory and to Indo-Pacific infectious disease surveillance partnerships through James Cook University.

My research has attracted international recognition, including the ESWI Young Scientist Award, the Sri Lankan Presidential Award for Scientific Publication, and nomination for the CSIRO Health & Biosecurity Research Excellence Award. My work on black swan susceptibility to avian influenza received national media coverage (ABC Science) and was published in Genome Biology.


Key Research Capabilities

Domain Expertise
Veterinary Pathology Anatomic pathology, diagnostic histopathology, gross and microscopic lesion interpretation, special staining, 1,000+ post-mortems (domestic and wildlife), ACVP Phase I passed
Genomics & Bioinformatics De novo genome assembly, transcriptomics (RNA-seq), metagenomics, variant calling, long-read sequencing (PacBio, Oxford Nanopore)
Virology & Immunopathology Avian influenza (HPAI/LPAI), Japanese encephalitis virus, SARS-CoV-2, lyssavirus; cell culture, viral infection models, molecular and immunopathology
High-Containment Operations PC3 facility trained and certified (ACDP); biosafety and approved arrangement certified
Computational Skills Python, R, Unix/Linux, Snakemake workflow manager, HPC job management (Slurm, PBSpro)
Environmental Biosecurity eDNA-based surveillance, environmental genomics, next-generation surveillance technologies
Research Leadership $520K+ in grants (lead/co-lead), 2 PhD students, Honours & undergrad supervision, ACDP Academic Board member
Engagement & Impact Cross-institutional collaborations (JCU, Deakin, UQ, international), NT capacity development, Indo-Pacific disease surveillance, media engagement, 6+ invited talks

Education

PhD (Pathobiology and Genomics) — University of Queensland, Australia (2021)
Thesis: “Swan genomics and transcriptomics give insights into the pathogenesis of highly pathogenic avian influenza in Australian Black swans”
Supported by UQ Postgraduate Scholarship and CSIRO Top-Up Scholarship (Australian Animal Health Laboratory)

Master of Philosophy (MPhil) — Veterinary Anatomic Pathology — University of Peradeniya, Sri Lanka (2017)
Specialisation: Wildlife diseases
Thesis: “Pathological Conditions in Indian Flying Fox (Pteropus medius) found dead in Peradeniya, Sri Lanka”
Joint student, MSc Veterinary Anatomic Pathology, Western College of Veterinary Medicine, University of Saskatchewan, Canada (one full semester of intensive pathology training, 2014)
Supported by International Development Research Centre Scholarship

Bachelor of Veterinary Science (Honours, Second Upper Division) — University of Peradeniya, Sri Lanka (2011)


Professional Credentials


Employment History

Senior Research Scientist — Pathology and Pathogen Biology Team

Australian Centre for Disease Preparedness (ACDP), CSIRO, Geelong
February 2023 – Present

Leading research in pathology and pathogen biology with a focus on emerging infectious diseases in avian and wildlife species. Integrating comparative genomics, transcriptomics, and anatomic pathology to understand host susceptibility and pathogenesis. Key activities include:

CSIRO Early Career Research Fellow (Postdoctoral Researcher) — Emerging Infectious Diseases

Australian Centre for Disease Preparedness (ACDP), CSIRO, Geelong
November 2021 – February 2023

Investigated the genesis and pathogenesis of viral diseases in avian species using genomics, transcriptomics, bioinformatics, virology, and immunopathology approaches.

PhD Candidature — Pathobiology and Genomics

University of Queensland / Australian Animal Health Laboratory, CSIRO
2018 – 2021

Assembled the first black swan reference genome and conducted comparative genomic and transcriptomic analyses to elucidate the molecular basis of HPAI susceptibility in Australian waterfowl. Trained in PC3 operations, cell culture, viral infection models, and HPC-based bioinformatics.

Research Assistant / Veterinary Pathologist

Department of Veterinary Pathobiology, University of Peradeniya, Sri Lanka
October 2013 – March 2017

Conducted routine diagnostic and research veterinary pathology across domestic and wild animal species. Performed over 1,000 post-mortem examinations. Prepared histological samples, interpreted gross and microscopic pathological lesions, and authored diagnostic reports. Contributed to multiple wildlife disease research publications.

Lecturer (Contract) — Veterinary Pathology

Department of Veterinary Pathobiology, University of Peradeniya, Sri Lanka
November 2012 – September 2013

Delivered practical teaching in gross and microscopic pathology to 2nd-year BVSc students. Supervised student projects, conducted post-mortem diagnosis of rabies, and taught histopathology laboratory methods.

Veterinary Internships


Institutional Service & Governance


Research Grants & Funding

Total competitive funding as lead or co-lead: $605,000+

# Grant Title Role Funder Period Amount
1 Improving capacity for emerging infectious diseases research and surveillance in the Indo-Pacific Co-lead James Cook University & CSIRO 2023–2025 $300,000
2 Artificial Intelligence for Mission Postgraduate Program Co-lead CSIRO 2022–2026 $155,000
3 Environmental DNA based next generation surveillance technology Project Lead CSIRO Environomics Future Science Platform 2022–2023 $35,000
4 High-performance desktop computer and Oxford Nanopore Technology sequencing platform (P2 Solo) Project Lead CSIRO CAPEX 2021 $30,000
5 Spatial transcriptomics capability development at ACDP: Lumpy skin disease pathogenesis investigation Project Lead IADZ 2024–present $30,000
6 Spatial transcriptomics to uncover the mechanisms of pathology of highly pathogenic avian influenza in commercial layers Project Lead IADZ 2024–present $25,000
7 Deakin University collaboration for JEV surveillance in Victorian waterbirds Project Lead VicHealth Ongoing $30,000

Submitted:

# Grant Title Role Funder Amount
1 AI4AI: A Robust AI-Driven Platform for Streamlining IHC-Based Avian Influenza Surveillance and Response Project Lead DAFF $300,000

Higher Degree Research Supervision

PhD Students (Current)

# Student Name University Project Title Role Period
1 R. P. James Cook University Bat flies as vectors for infectious agent transmission Co-supervisor 2023–2027
2 G. M. University of Queensland Long COVID and the nasal transcriptome Co-supervisor 2023–2026

Honours Students

# Student Name University Project Title Role Year
1 N. B. Deakin University Machine learning for peptide prediction Principal Supervisor 2024
2 A. D. Deakin University JEV surveillance in Australian wild birds Co-supervisor 2024

Undergraduate & Short-term Supervision


Publications in Peer-Reviewed Journals

For a complete and current list of publications, see Google Scholar or ORCID.

2025

  1. Ahmed, K. A., Karawita, A. C., Klein, M. J., Mincarelli, L. F., Secondini, B., Satta, G., … Hardy, C. M. (2025). Complete mitochondrial genomes of Culicoides brevitarsis and Culicoides imicola biting midge vectors of Bluetongue Virus. Mitochondrial DNA Part B, 10(1): 67–71. https://doi.org/10.1080/23802359.2024.2447750

  2. Gofton, A. W., Short, M., Michie, M., Gallaway, D., Smith, I., Blasdell, K., Karawita, A. C., Klein, M. J., Herbert, C. A., Barker, S. C. (2025). Exploring Borrelia in Australia: Isolation, genomic characterisation, and host and vector associations. Ticks and Tick-borne Diseases, 16(4): 102505.

  3. McNamara, B. J., Cornish, J., Blasdell, K. R., Athan, E., Clarke, N. E., Pe, T., Hussain, M. A., Muleme, M., Tay, E. L., Dunn, M., Boyd, V., Karawita, A. C., O’Brien, D. P. (2025). Mycobacterium ulcerans in possum feces before emergence in humans, Australia. Emerging Infectious Diseases, 31(3): 569.

2024

  1. Sinclair, J. E., Vedelago, C., Ryan, F. J., Carney, M., Redd, M. A., Lynn, M. A., Grubor-Bauk, B., Cao, Y., Henders, A. K., Chew, K. Y., Gilroy, D., Greaves, K., Labzin, L., Ziser, L., Ronacher, K., Wallace, L. M., Zhang, Y., Macauslane, K., Ellis, D. J., Rao, S., Burr, L., Bain, A., Karawita, A. C., Schulz, B. L., Li, J., Lynn, D. J., Palpant, N., Wuethrich, A., Trau, M., Short, K. R. (2024). Post-acute sequelae of SARS-CoV-2 cardiovascular symptoms are associated with trace-level cytokines that affect cardiomyocyte function. Nature Microbiology, 9(12): 3135–3147.

Preprints:

2015–2023 (published)

  1. Karawita, A. C., Cheng, Y., Chew, K. Y., Challagulla, A., Kraus, R., Mueller, R. C., et al. (2023). The Swan Genome and Transcriptome, It Is Not All Black And White. Genome Biology, 1(24). https://doi.org/10.1186/s13059-022-02838-0

  2. Zhu, Y., Chew, K. Y., Wu, M., Karawita, A. C., McCallum, G., et al. (2022). Ancestral SARS-CoV-2, but not Omicron, replicates less efficiently in primary pediatric nasal epithelial cells. PLOS Biology, 20(8): e3001728. https://doi.org/10.1371/journal.pbio.3001728

  3. Lu, J., Yang, Z., Karawita, A. C., Bunte, M., Chew, K. Y., Pegg, C., Mackay, I., Whiley, D., Short, K. R. (2021). Limited evidence for the role of environmental factors in the unusual peak of influenza in Brisbane during the 2018–2019 Australian summer. Science of The Total Environment, 776, 145967. https://doi.org/10.1016/j.scitotenv.2021.145967

  4. Hulme, K. D., Karawita, A. C., Pegg, C., Bunte, M. J. M., Bielefeldt-Ohmann, H., Bloxham, C. J., Van den Hoecke, S., Setoh, Y. X., Vrancken, B., Spronken, M., Steele, L. E., Verzele, N. A. J., Upton, K. R., Khromykh, A. A., Chew, K. Y., Sukkar, M., Phipps, S., Short, K. R. (2021). A paucigranulocytic asthma host environment promotes the emergence of virulent influenza viral variants. eLife, 10:e61803. https://doi.org/10.7554/eLife.61803

  5. Karawita, A. C., Tong, M. Z. W., Short, K. R. (2019). A delicate balancing act: immunity and immunopathology in human H7N9 influenza virus infections. Current Opinion in Infectious Diseases.

  6. Une, Y., Yamazaki, M., Morimoto, M., Kametani, F., Parker, D., Karawita, A., Scott, S. J., Yamazaki, M., Kametani, S., Parker, K. (2018). Cerebral amyloid angiopathy and comparative analysis of amyloid-β protein in birds. bioRxiv, p.486340.

  7. Karawita, A. C., Himsworth, C. G., Rajapakse, R. P. V. J., Bollinger, T. K., Gunawardena, P. de S. (2016). Toxocara pteropodis in Indian flying fox Pteropus medius in Sri Lanka. Journal of Wildlife Diseases, 53(2): 414–416. http://dx.doi.org/10.7589/2016-07-170

  8. Gunawardena, P. de S., Marston, D. A., Wise, E. L., Karawita, A. C., Breed, A. C., McElhinney, L. M., Johnson, N., Banyard, A. C., Fooks, A. R. (2016). Lyssavirus in Indian Flying foxes, Sri Lanka. Emerging Infectious Diseases, 22(8): 1456–1459.

  9. Karawita, A. C., Perera, B. V. P., Perera, G. I. S., de Silva, M. D. S., Jayaweera, W. R., Himsworth, C. G., Bollinger, T. K., Gunawardena, G. S. P. de S. (2016). Calodium hepaticum in Jungle Cats (Felis chaus) in Sri Lanka. Journal of Wildlife Diseases.

  10. Karawita, A. C., Rajapakse, R. C., Amarasiri, P. G. I. D., Ramanayake, R. M. T. M., Jayaweera, W. R., Bollinger, T. K., Gunawardena, G. S. P. De S. (2015). Cecocolic Intussusception in an Asian Elephant (Elephas maximus) in Sri Lanka Associated with Chronic Hepato-Intestinal Schistosomiasis: First Case Report. International Journal of Applied Sciences and Biotechnology, 3(4), 744–746.


Conference Presentations & Published Abstracts

Oral Presentations

  1. Karawita, A. C., Cheng, Y., Tong, M., Mueller, R., Bielefeldt-Ohmann, H., Chew, K., Bruxner, T., Kraus, R., Challagulla, A., Lowther, S., McAuley, A., Smith, J., Kuo, R., Baker, M., Burt, D., Short, K. (2020). Comparative genomics and transcriptomics help unravel why Australian Black Swans are uniquely susceptible to highly pathogenic avian influenza (HPAI). European Scientific Working Group in Influenza (ESWI) 7th Conference, Spain.

Poster Presentations & Published Abstracts

  1. Karawita, A. C., Blasdell, K., Blanch-Lazaro, B., Challagulla, A., Selleck, P., McGinness, H., McLelland, J., Su, J., Suen, W., Wong, F., Wille, M., Horwood, P., Klaassen, M., Short, K., Hickson, R. (2023). Assembly and analysis of the native Australian ibis genomes to understand the species’ competency for infectious diseases. International Congress of Genetics and Genomics, Melbourne.

  2. Karawita, A. C., Cheng, Y., Bielefeldt-Ohmann, H., Burt, D., Short, K. (2018). Highly Pathogenic Avian Influenza in Australian Black Swans (Cygnus atratus). Australian Ornithological Society Conference.

  3. Karawita, A. C., Jagatheesan, M., Koizumi, N., Gamage, C. D., Bollinger, T. K., Gunawardena, P. de S. (2016). Infectious Agents in Indian Flying Fox (Pteropus medius) in Sri Lanka. 4th International One Health Congress & 6th Biennial Congress of the International Association for Ecology and Health, Melbourne.

  4. Perera, B. V. P., Karawita, A. C., Jinadasa, H. R. N., Rajapakse, R. P. V. J., Bollinger, T. K., Gunawardena, G. S. P. de S. (2016). Zoonotic Pathogens in Free Ranging Wild Mammals in Sri Lanka. One Health Eco Health 2016, Melbourne.

  5. Jagatheesan, M., Karawita, A. C., Muthusinghe, D. S., Lokugalappatti, L. G. S., Bollinger, T. K., Gunawardena, P. De S., Koizumi, N., Gamage, C. D. (2016). Presence of Pathogenic Leptospira sp. in Indian Flying foxes in Sri Lanka. 4th Annual Sessions of The Sri Lanka Society for Microbiology, Kandy.

  6. Perera, B. V. P., Karawita, A. C., Gunawardena, G. S. P. De S., Rajapakse, R. P. V. J. (2015). Presence of zoonotic pathogens in free ranging wild cats in Sri Lanka. Symposium on Wild Cats of South Asia: Past and Present, Sri Lanka.

  7. Amarasiri, P. G. I. D., Ramanayake, R. M. T. M., Karawita, A. C., Perera, K. U. E., Jayaweera, W. R., Gunawardena, G. S. P. de S. (2015). Bovine Tuberculosis: A Histopathological Study Of The Lung Lesions. Peradeniya University International Research Sessions, Sri Lanka.

  8. Ramanayake, R. M. T. M., Amarasiri, P. G. I. D., Karawita, A. C., Jayaweera, W. R., Obayashi, Y., Hiko, T., Gunawardena, G. S. P. de S. (2015). Changes in the demographic pattern of animal rabies in the Central province of Sri Lanka. Conference on Sri Lanka Japan Collaborative Research.

  9. Ramanayake, R. M. T. M., Amarasiri, P. G. I. D., Karawita, A. C., Jayaweera, W. R., Obayashi, Y., Hiko, T., Gunawardena, G. S. P. de S. (2015). Sensitivity and Specificity of the Negri Body Test using Sellers’ Stain in the Diagnosis of Rabies in Dogs. Conference on Sri Lanka Japan Collaborative Research.

  10. Karawita, A. C., Ariyarathna, H. M. H. S., Fernando, W. D. D., Nizananthan, K., Amarasinghe, A. A. A. W. K., de Silva, L. N. A., Rajapakse, R. P. V. J., Jayaweera, W. R., Gunawardena, G. S. P. De S. (2013). Arterial thromboembolism caused by Strongylus vulgaris larvae migration in a thoroughbred horse — a case report. Scientific Sessions — Sri Lanka Veterinary Association. (Poster)

  11. Karawita, A. C., Ariyarathna, H. M. H. S., Jayaweera, W. R., Obayashi, Y., Umemura, T., Tamashiro, H., Gunawardena, G. S. P. De S. (2013). Presence of Rabies and its Distribution among Animals in the Central Province in Sri Lanka. Conference on Sri Lanka Japan Collaborative Research.

  12. Ariyarathna, H. M. H. S., Karawita, A. C., Jayaweera, W. R., Gunawardena, G. S. P. De S. (2012). A Retrospective Study on Necropsy Findings in dogs with renal disease. Peradeniya University Research Sessions.

  13. Ariyarathna, H. M. H. S., Mallawa, M. R. C. K., Bandara, T. P. M. S. D., Jayaweera, W. R., Karawita, A. C., Bandara, A. M. R., Silva, I. D., Arulkanthan, A., Gunawardena, G. S. P. De S. (2013). Canine Urinalysis with Dipsticks Designed for Humans: Uses and Limitations. Scientific Sessions — Sri Lanka Veterinary Association.

  14. Abeygunawardena, H. E., Silva, I. D., De Silva, D. D. N., Dangolla, A., Weerasinghe, J. U., Hewapathirana, I. S., Bandara, A. M. R., Gunasekara, W. U. S., Karawita, A. C. (2010). An embolectomy in the left external iliac artery in a paraplegic dog. Peradeniya University Research Sessions, Vol. 15, 193–195.


Invited Talks

  1. Invited speaker — Wildlife research symposium for University of Melbourne final year Veterinary students, 26 October 2023
  2. Invited speaker — International Plant and Animal Genome Conference / PAG 30, San Diego, USA, 13–18 January 2023
  3. Invited speaker — 2nd Sino-Australia Symposium, James Cook University, July 2022
  4. Invited speaker — “Molecular Horizons”, University of Wollongong, 30 June 2022
  5. Invited speaker — 3rd Ecosystem Change and Population Health (ECAPH) Virtual Symposium, QUT, June 2022
  6. Invited speaker — STEM student visit, Western Heights College, 9 November 2022
  7. Invited speaker — ANZCVS Science Week, Gold Coast, 24 July 2026 — “Molecular meets morphology! An introduction to ISH and spatial transcriptomics”

Awards & Recognition

  1. CSIRO Health & Biosecurity “Research Excellence Award” — Nominated (2023) for The Swan Genome and Transcriptome, It Is Not All Black And White (Genome Biology)
  2. European Scientific Working Group on Influenza (ESWI) Young Scientist Award — October 2020
  3. Presidential Award for Scientific Publications — National Research Council of Sri Lanka, November 2018, for “Lyssavirus in Indian Flying Foxes in Sri Lanka” (Emerging Infectious Diseases)
  4. Sri Lankan President’s Award for Scientific Publication — November 2018
  5. University of Queensland Postgraduate Scholarship — Living stipend and tuition fee, 2018–2022
  6. CSIRO Top-Up Scholarship — Australian Animal Health Laboratory, 2018–2022
  7. Taronga Conservation Society Australia Scholarship — Wildlife Health and Pathology Short Course, February 2019
  8. International Development Research Centre (IDRC) Scholarship — MPhil Research, 2013–2017

Applied Impact & Capacity Development


Media Coverage

  1. “Bird flu could wipe out Australian black swans; gene study reveals” — ABC Science, 23 January 2023
  2. “Are bin chickens spreading Japanese encephalitis?” — Science in Public, 19 July 2023

Technical Skills

Category Detail
Pathology Gross and microscopic pathological examination; diagnostic histopathology; H&E and special staining; sample trimming, processing, embedding; post-mortem examination (domestic and wild species); rabies diagnosis
Genomics De novo genome assembly; transcriptomics (RNA-seq); metagenomics; long-read sequencing (PacBio HiFi, Oxford Nanopore); QIIME2 microbiome analysis
Virology Cell culture; viral infection models; molecular and immunopathology; influenza, JEV, SARS-CoV-2, lyssavirus
Programming Python, R, Unix/Linux shell scripting
Workflow & HPC Snakemake; Galaxy; Slurm; PBSpro; high-performance computing cluster management
Biosafety PC3 facility operations (ACDP); Approved Arrangement and Biosafety Awareness certified
General MS Office (Word, Excel, PowerPoint)

Professional Training (Selected)

Year Training Provider
2022 QIIME2 microbiome analysis AustralianBioComms
2020 Variant Calling and De Novo Genome Assembly with PacBio HiFi Reads (webinar) PacBio
2019 Genome Assembly Using Galaxy Workshop QFAB / University of Queensland
2019 Assembly of long-read sequencing (2-day workshop) QFAB Australia
2019 Approved Arrangement and Biosafety Awareness Certificate Dept. of Agriculture and Water Resources, Australian Government
2019 Physical Containment Facility 3 (PC3) Training AAHL / CSIRO, Geelong
2019 Wildlife Health and Pathology Short Course Taronga Zoo, Sydney
2018 High-Performance Computing (HPC) Training Research Computing Centre, University of Queensland
2018 Software Carpentry (Unix, Python, R) University of Southern Queensland
2016 PC3 Induction — Biosciences Laboratory Dept. of Agriculture and Fisheries, Queensland
2014 Intensive training in veterinary pathology (1 semester) University of Saskatchewan, Canada — Dr C. G. Himsworth (Dipl. ACVP), Dr H. Philibert, Dr T. K. Bollinger
2013 Introduction to Laboratory Animal Science Sri Lanka Association for Laboratory Animal Science

Training & Workshops Delivered (as Resource Person)

  1. “Wildlife Health & Management Training Program for Wildlife Officers” — Elephant Transit Home, Uda-walawe, Sri Lanka, August 2014
  2. “Workshop on Collection of Brain Samples from Animals for Diagnosis of Rabies” — Dept. of Animal Production & Health / Dept. of Wildlife Conservation / University of Peradeniya / Sri Lanka Wildlife Health Centre, December 2013
  3. “Disease Surveillance in Wildlife: Procedures for Collection, Storage and Transport of Laboratory Samples” — Dept. of Animal Production & Health / Dept. of Wildlife Conservation / University of Peradeniya / Sri Lanka Wildlife Health Centre, February 2013